Coordination topics for the reorganized International Triticeae Mapping Initiative (ITMI)

ITMI should be a comprehensive service to facilitate progress in genomic research for Triticeae species. The full spectrum of necessary activities and tools comprise at least the following topics. ITMI should provide coordination for these as deemed necessary, enlisting or supplementing existing efforts. The person (planning chair) designated (with email contact information) for each topic has volunteered or will be requested to develop by December 1, 2000, for an Overall Planning Committee, a specific coordination plan and propose a coordinator and workgroup to carry out that coordination. The planning chair will seek guidance for this task from others directly or as volunteers.

1. EST production and library collation

With the welcome proliferation of national EST projects focusing on wheat and barley, there is the potential for much duplication in sequencing effort and library coverage. A lack of high-quality libraries is a bottleneck for several labs with sequencing facilities. A web-based clearinghouse function presenting descriptive information about libraries in use for ESTs and by whom could help avoid unnecessary duplication of effort and help identify available libraries for labs that need them.

Olin Anderson, USDA-ARS-WRRC, Albany CA USA

2. Bioinformatics: provide information about and access to tools; organize annotation of ESTs

It has been clearly pointed out that a bottleneck in analysis and utilization of ESTs is incomplete information about them. At least two things would be useful (i) a clearinghouse website with description and access information on relevant software applications in use for EST analysis and (ii) a coordinated approach to annotation of sequences. A roster of expertise could be maintained and tapped for annotation assistance.

David E. Matthews, USDA-ARS-CBCG, Cornell University, Ithaca NY USA

3. Large-insert libraries (e.g., hexaploid wheat BACs)

A clearinghouse webpage with status and links to resources.

Sylvie Cloutier, A&AFC, Winnipeg, MB CANADA

4. Functional markers (SNPs, SSRs)

A clearinghouse webpage with status and links to resources.

Wheat: Peter J. Sharp, University of Sydney, Sydney NSW AUSTRALIA
Barley: Gary Muehlbauer, University of Minnesota, St. Paul MN USA

5. Organizing contiguous genome coverage for physical mapping (contiging)

Wheat: Jan Dvorak, University of California, Davis CA USA
Barley: Andy Kleinhofs, Washington State University, Pullman WA USA

6. Linkage maps and QTLs

Coordination by each homoeologous chromosome group and/or genome remains relevant. Each group should have (or contribute to) a webpage, linking relevant progress for that group. This would replace the annual hard-copy poster or document that has been the current practice.

Wheat and rye: Rudi Appels, Div. of Plant Industry, CSIRO, Canberra ACT AUSTRALIA
Barley: Patrick Hayes, Oregon State University, Corvallis OR USA

7. Arraying, identification of singletons

Development of a public Trit-chip needs planning for process, contents, and funding.

Wayne Powell, DuPont Company, Newark DE USA

8. Genetic stocks and gene symbols

It is recognized that there are barley and wheat stock collections and gene symbol committees, but to have this information consolidated for the entire community would be advantageous.

Genetic stocks: Bikram S. Gill, Kansas State University, Manhattan KS USA
Gene symbols: Katrien Devos, John Innes Centre, Norwich UK

9. Developing areas (e.g., induced mutation, reverse genetics)

New efforts would be facilitated by communication and pooling of resources.

Beat Keller, University of Zürich, Zürich SWITZERLAND

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